We will conduct phenotyping in a three-tiered approach in order to create a comprehensive and data-rich annotation of the mutants and utilise the specialist expertise of the European zebrafish research community:
- Initial phenotyping for morphology and behaviour at day 5 will be carried out by the laboratories that generate the mutants (mainly by GRL, UK).
- Second-level phenotyping will be carried out for a subset of mutants with first-level phenotypes, as well as on selected mutants from earlier screens, as well as morphants or pharmacologically-treated animals. It will include behavioural phenotyping (by CNRS, France, KIT-G, Germany, and UZH, Switzerland), 3D / 4D imaging (CNRS and KIT-G) and transcript Amounting (mainly by GRL).
- Third-level phenotyping will include shelf screens for additional phenotypes in which all project partners as well as external researchers will be invited to participate, contributing their assays; as well as in-depth phenotyping and analysis of disease-relevant pathways in the home labs of the participants.
(Work Package Leader: CNRS)